Background:

Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is the most frequently encountered of the group and the most toxic one. Aflatoxins can be found mainly in cereals, nuts, cottonseed and human blood.

Aflatoxin B1 is a potent human carcinogen, and may contribute to human liver cancer.

Manufacturer	Meizheng
Trademark	ToxinFast®
Order No.	HEM0396
Analyte	Aflatoxins B1
Sample Type	Corn, rice, wheat, beans, peanut, peanut butter, edible oil and food or feed made of grain
Methodology	Indirect competitive enzyme-labeled immunoassay.
Packaging	96 Wells
Storage	The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf life	The validity for this kit is 12 months
Lead time	Shipped within 5-12 working days.
Cross Reactivity	AFB1 100% AFB2 38% AFG1 27% AFG2 4% AFM1 20% AFM2 <1%

 Calculate Result:

Please use the professional analysis instruments of Elisa kit for calculation, which is more accurate and fast to get result for kinds of samples

Sensitivity, accuracy and precision

Sensitivity:           10ng/kg

Limit of detection

Rice flour…………………………………… 120ng/kg

Milk………………………………………………………………………… 50ng/kg

Percent Recovery, %

Rice flour and milkl: 90±15%

Precision (CV %):

Intra-lab assay: CV%≤10%

Provided Materials and Reagent:

Component	96 wells
Antigen coated plate	12 test strips of 8 wells each
5 vials of Standard	1mL each
Enzyme Conjugate	6mL
Antibody Solution	6mL
Substrate A	6mL
Substrate B	6mL
Concentrated sample diluting buffer	40mL
Concentrated Wash Buffer (10×)	40mL
Package Insert	1
Plate Cover Sheet	2
Testing Report	1

 Note: The concentrations of the standards are 0μg/kg, 0.03μg/kg, 0.12μg/kg, 0.48μg/kg and 2μg/kg respectively.

Materials Required But Not Provided

1. Devices:

---- Microwell plate Reader (450nm/630nm)

---- Oscillator

---- Vortex

---- Centrifuge /funnel

---- Balance (sensibility: 0.01g)

---- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)

----Multi-channel pipette (30µL-300µL)

---- Pipette/ triangular flask/ beaker

---- Rubber suction bulb

---- Centrifuge tube (7mL, 50mL)

---- Quantitative analysis filter paper

2. Reagent:

---- Methanol (AR)

---- Deionized water

---- Acetonitrile (AR)

---- Concentrated sulfuric acid (18mol/L)

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.